Open Access Publications
- Nanna S. Mikkelsen∙ Sujan Ravendran∙ Amalie D. Broksø ∙ Thorkild Terkelsen Martin Kristian Thomsen ∙ Rasmus O. Bak Orthogonal CRISPR systems for targeted integration and multiplex base editing enable nonviral engineering of allogeneic CAR-T cells.
Molecular Therapy 2025 doi: https://doi.org/10.1016/j.ymthe.2025.08.032
READ THE FULL ARTICLE - Wagner DL, Ostendorf L. New dawn of cellular therapies in autoimmune diseases. Molecular Therapy Methods & Clinical Development, 2023, DOI:https://doi.org/10.1016/j.omtm.2023.101141
READ THE FULL ARTICLE - Kath J, Franke C, Drosdek V, Du W, Glaser V, Fuster-Garcia C, Stein M, Zittel T, Schulenberg S, Porter CE, Andersch L, Künkele A, Alcaniz J, Hoffmann J, Abken H, Abou-el-Enein M, Pruß A, Suzuki M, Cathomen T, Stripecke R, Volk HD, Reinke P, Schmueck-Henneresse M, Wagner DL. Integration of ζ-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells. BioRxiv 2023. DOI: https://doi.org/10.1101/2023.11.10.565518
READ THE FULL ARTICLE - Kath J, Franke C, Drosdek V, Du W, Glaser V, Fuster-Garcia C, Stein M, Zittel T, Schulenberg S, Porter CE, Andersch L, Künkele A, Alcaniz J, Hoffmann J, Abken H, Abou-el-Enein M, Pruß A, Suzuki M, Cathomen T, Stripecke R, Volk HD, Reinke P, Schmueck-Henneresse M, Wagner DL. Integration of ζ-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells. Blood 2023. DOI: https://doi.org/10.1182/blood.2023020973
READ THE FULL ARTICLE - Du, W., Noyan, F., McCallion, O., Drosdek, V., Kath, J., Glaser, V., Fuster-Garcia, C., Yang, M., Stein, M., Franke, C., Pu, Y., Weber, O., Polansky, J.K., Cathomen, T., Jaeckel, E., Hester, J., Issa, F., Volk, H.D., Schmueck-Henneresse, M., Reinke, P., Wagner, D.L. Gene editing of CD3 epsilon to redirect regulatory T cells for adoptive T cell transfer. Mol Ther. 2025;33(3):997-1013. PMC: PMC11897813. DOI: 10.1016/j.ymthe.2025.01.045
READ THE FULL ARTICLE - Nitulescu A-M, Du W, Glaser V, Kath J, Aird EJ, Cullot G, Greensmith R, Steengaard Mikkelsen N, Stein M, Rasmus Bak R, Kaminski M, Corn JE, Wagner DL. Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cells. Molecular Therapy Nucleic Acids 2025. DOI: https://doi.org/10.1016/j.omtn.2025.102568
READ THE FULL ARTICLE - Cullot G, Aird EJ, Schlapansky MF, Yeh CD, van de Venn L, Vykhlyantseva I, Kreutzer S, Mailänder D, Lewków B, Klermund J, Montellese C, Biserni M, Aeschimann F, Vonarburg C, Gehart H, Cathomen T & Corn JE Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations. Nature Biotechnology 2025. DOI: https://doi.org/10.1038/s41587-024-02488-6
READ THE FULL ARTICLE - Ala Dibas, Manuel Rhiel, Vidisha Bhavesh Patel, Geoffroy Andrieux, Melanie Boerries, Tatjana I Cornu, Jamal Alzubi, Toni Cathomen Cell-Based Models of ‘Cytokine Release Syndrome’ Endorse CD40L and Granulocyte–Macrophage Colony-Stimulating Factor Knockout in Chimeric Antigen Receptor T Cells as Mitigation Strategy. Cells 2023. DOI: 10.3390/cells12212581
READ THE FULL ARTICLE - Benjamin K., Bhandari A., Kepple JD., Qi R., Shang Z., Xing Y., An Y., Zhang N., Hou Y., Crockford TL., McCallion O., Issa F., Hester J., Tillmann U., Harrington HA., Bull KR. Multiscale topology classifies cells in subcellular spatial transcriptomics. Nature 2024. DOI: 10.1038/s41586-024-07563-1
READ THE FULL ARTICLE - Martini S, Drzeniek N, Stark R, Koller MR, Du W, Reinke S, Ort M, Hardt S, Kotko I, Kath J, Schlickeiser S, Geißler S, Wagner DL, Krebs AC, Volk HD. Long-term in vitro maintenance of plasma cells in a hydrogel-enclosed human bone marrow microphysiological 3D model system. Biofabrication 2024 DOI: https://doi.org/10.1088/1758-5090/ad5dfe
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